Australia have been left with just one player in the second round of Wimbledon after Daria Gavrilova's loss capped off a disappointing opening round.
All six Australian men have been eliminated after Thanasi Kokkinakis, Bernard Tomic and Jordan Thompson joined Nick Kyrgios, John Millman and Andrew Whittington as first-round casualties.
Gavrilova experienced more grand slam despair with a drama-charged three-set defeat, joining Ash Barty on the loser's list.
The new Australian No.1 and tournament's 20th seed bowed out with a 6-4 2-6 10-8 loss to Croatian qualifier Petra Martic.
Despite the 23-year-old's rapid rankings rise, Gavrilova has won just four grand slam matches in her past five majors since charging into the fourth round at the 2016 Australian Open.
Tomic started the rot on Tuesday with an insipid display against Mischa Zverev.
Tomic, a former quarter-finalist at Wimbledon, had thrashed Zverev only last week in Eastbourne, but slumped to a 6-4 6-3 6-4 defeat in just 84 minutes.
Tomic complained of hurting his back in the warm-up but even his victorious opponent was left surprised by the ease of his passage through to the second round.
Kokkinakis went down to Juan Martin del Potro 6-3 3-6 7-6 (7-2) 6-4 after the 29th-seeded Argentine secured victory with his seventh match point.
Kokkinakis fought valiantly and was praised for his courageous effort, but was no match for the former US Open winner.
Thompson's strong grasscourt form came to an abrupt halt with a disappointing loss.
Spanish 25th seed Albert Ramos-Vinolas beat the Sydneysider 6-4 6-4 7-6 (7-4) just a fortnight after Thompson stunned world No.1 Andy Murray in the opening round at Queen's.
It leaves Arina Rodionova as the only remaining Australian after the qualifier staved off seven match points to secure her maiden grand slam singles victory.