The lyrics, penned by songwriter Barry Mason, describe the murder of a woman by her boyfriend after seeing her with another man.
The February 2023 ban came after allegations were made in a BBC documentary at the time of a “toxic” culture at the WRU that included sexism, racism and homophobia.
“Delilah will not feature on the playlist for choirs for rugby internationals at Principality Stadium,” a Principality Stadium statement said.
“The WRU removed the song from its half-time entertainment and music playlist during international matches in 2015. Guest choirs have also more recently been requested not to feature the song during their pre-match performances and throughout games.”
Wales enter the 2024 Six Nations as a team in transition, with 21 of their squad having 20 caps or fewer following a slew of retirements and further injuries. They face Scotland in Cardiff in their opening match on Saturday.