England lock Joe Launchbury has lost his race to be fit for the first Test against South Africa on as coach Eddie Jones again named Mike Brown on the wing.
Jones kept with the positional switch he made for last month's 63-45 loss to the Barbarians at Twickenham when he swapped Elliot Daly from the left wing to fullback and stuck Brown out wide.
Launchbury had trained apart from the rest of the squad at the coastal resort of Umhlanga this week as he battled with a calf problem and will have his place taken in the second row by Nick Isiekwe, who gets his first start for England
Isiekwe is one of six players from English champions Saracens in the starting line-up.
Kyle Sinckler got the nod over Harry Williams at tighthead in place of Dan Cole, who has been rested for the tour, while Tom Curry was chosen at No.7 for only his second cap.
Billy Vunipola returns after more than a year without international rugby.
There had also been concern over Vunipola's latest injury - a persistent hamstring problem he had been playing through in recent weeks for Saracens but he has been selected to add to his 34 caps.
Uncapped New Zealand-born loose forward Brad Shields is likely to get a first cap off the bench even though his selection has sparked controversy.
World Rugby vice-chairman Augustin Pichot is among those who have been critical of rugby's eligibility rules which allows athletes such as Shields to qualify to play for Jones' team through his English-born parents.
"We have had a really good week in preparation for this weekend and we are pleased with the way the squad has come together," said Jones, who has opted to train in sunnier climes and only travel up to high altitude Johannesburg on the eve of the Ellis Park Test.
"We want to do something special on this tour and the players are all incredibly excited about this challenge and opportunity we have."
ENGLAND: Elliot Daly, Jonny May, Henry Slade, Owen Farrell (capt), Mike Brown, George Ford, Ben Youngs, Billy Vunipola, Tom Curry, Chris Robshaw, Nick Isiekwe, Maro Itoje, Kyle Sinckler, Jamie George, Mako Vunipola. Replacements: Luke Cowan-Dickie, Joe Marler, Harry Williams, Brad Shields, Nathan Hughes, Ben Spencer, Piers Francis, Denny Solomona.