Injury and illness have hit Wallabies camp in Florence, forcing Nick Frost out of Dave Rennie's inexperienced side to face Italy.
The lock has a minor ankle issue and is battling sickness, set to be replaced by Darcy Swain with Cadeyrn Neville moving onto the bench.
Fullback Jock Campbell also missed Friday's captain's run photo as he too battles an illness that could keep him out of a second straight start in the No.15.
They're more disruptions for coach Dave Rennie, who doesn't believe he's rolling the dice after picking the least experienced Wallabies line-up of his 32-Test tenure for a hiding-to-nothing showdown with the Azzurri.
Rennie will blood X-factor Mark Nawaqanitawase in Florence on Sunday morning (AEDT) while the winger's NSW Waratahs teammate Ben Donaldson could also earn his first cap after being named on the bench as the reserve five-eighth.
All up, Rennie's 23-man squad features eight players with less than 10 Tests each under their belts.
Under pressure to improve on an unflattering 39 per cent winning strike rate since succeeding Michael Cheika in 2020, Rennie risks becoming the first Wallabies coach to lose to Italy in 19 Tests against the Azzuri, who impressed when beating Samoa 49-17 last weekend.
But the New Zealander is bravely laying his reputation on the line for the greater good of the Wallabies, intent on building depth ahead of next year's Rugby World Cup in France - come what may.
"Every game is a challenge over here. We knew that," Rennie said when asked if he was making a selection gamble with his relatively greenhorn outfit.
"We were playing five Tests, which no one else is doing. You can't churn out the same side five weeks in a row and expect to be at our best late in the tour so we've got confidence in the group.
"We've got really good depth. When you look at the team, it's a good side. We expect them to front. They've prepared really well so far."
To be led by first-time Test skipper Allan Alaalatoa, who will become the Wallabies' 85th captain, Rennie's side also includes first starts this tour for playmaker Noah Lolesio, halfback Jake Gordon, flankers Ned Hanigan and Fraser McReight, France-based lock Will Skelton, hooker Folau Fainga'a and prop Matt Gibbon.
McReight is deputising for champion former Wallabies captain Michael Hooper, while outside back Jordan Petaia, and back-rower Langi Gleeson, hooker Lachlan Lonergan and lock Darcy Swain - who only earned a call-up after Matt Philip suffered an ACL tear the day the Wallabies flew out for Europe - will don the gold jumper for the first time on the tour after winning bench spots.
But Nawaqanitawase has the best tale for his grand children.
The 22-year-old had just landed in Auckland last month for a holiday with his girlfriend when he received a call from Rennie asking him to return to Sydney to join the spring tourists.
Now Nawaqanitawase is making his Test debut with a chance to press his claims for a World Cup berth.
"He's had a hell of a season, was excellent for Australia A in Fiji and Japan," Rennie said.
"He's a big man so aerially outstanding going forward and under the high ball.
"Has got the ability to beat defenders, got post-tackle presence, made big shifts defensively, kicks the ball well.
"He's a big man and generally we've got a small backline so it gives us a point of difference."
WALLABIES: Jock Campbell, Mark Nawaqanitawase, Len Ikitau, Hunter Paisami, Tom Wright, Noah Lolesio, Jake Gordon, Pete Samu, Fraser McReight, Ned Hanigan, Will Skelton, Darcy Swain, Allan Alaalatoa (capt), Folau Fainga'a, Matt Gibbon. Reserves: Lachlan Lonergan, Tom Robertson, Taniela Tupou, Cadeyrn Neville, Langi Gleeson, Tate McDermott, Ben Donaldson, Jordan Petaia.