Australia's 4x100m - 34 points freestyle relay team of Ellie Cole, Maddison Elliott, Katherine Downie and Jacqueline Freney blitzed the field to win gold and set a new world record at the end of Day Five.
The win gives Freney her fifth gold of the games, Cole her second and makes 13-year-old Elliott - already Australia's youngest Paralympics medallist after her bronze in the 400m S8 freestyle and silver in 50m S8 freestyle - now the youngest to have won gold.
Australia finished in 4:20.39 with the United States claiming silver and Britain taking the bronze.
Cole began the race and stayed in contention with America's Susan Beth Scott, handing over to Elliott just 0.2 seconds behind.
By the time Elliott had finished her leg the Aussies were six-and-a-half seconds ahead and this was extended to over nine seconds by Downie.
Freney, who earlier in the S7 freestyle 100m, then had to maintain the lead against British and American swimmers in higher classes and she did just that as Australia finished in a time of 4:20.39 - still over four seconds in front.
"These girls swim out of their skin for Australia, I didn't want to let them down at all," Freney said after the race.
"I gave it my all. We ended up with a world record and got a gold medal to boot, I couldn't be prouder."
Freney said she was overwhelmed to get her fifth gold -- with more on the cards.
"It's absolutely incredible, I can't explain how happy I am. The meet has gone to plan, every bit of it.
"I take every race in my stride. I've got eight events and my main one hasn't even started yet, so these are just bonuses to me, the icing on the cake."
The relay gold was a double victory for Australia after its men's team won the same event on Sunday.
Freney, who has cerebral palsy, seized the S7 100m freestyle title earlier on Monday. She set a new world record in the SM7 200m individual relay, and won the S7 50m butterfly and S7 100m backstroke last week.
The Australian won three bronze medals at the Beijing Paralympics in 2008.