The NRL fines issued to Latrell Mitchell and Josh Addo-Carr as a result of social-distancing breaches will stand after their appeal was denied.
The pair attempted to challenge the $20,000 fines - resulting from their weekend camping in Taree, NSW, in April - for bringing the game into disrepute.
Mitchell and Addo-Carr will also have additional $30,000 fines and one-match bans hanging over their heads for 12 months, after parts of their punishment fell under a suspended sentence.
In addition, they were fined $1000 by the NSW Police for social-distancing breaches.
At the time, Addo-Carr claimed he took family to Mitchell's farm to "put smiles on their faces" after they had been going through a tough time.
The incident - along with Nathan Cleary's infamous TikTok video - prompted questions over the return of the NRL from the COVID-19 shutdown.
The NSW State of Origin duo are due in court next month over firearms offences stemming from the weekend, after posting videos and photos of their get-together.