The goalkeeper was forced off at half-time of United's 3-2 win at Southampton after colliding with the post when failing to save James Ward-Prowse's free-kick.
Issuing an update on Tuesday, Ole Gunnar Solskjaer said "everyone's held their hand up and is available for selection", with De Gea among them - and the number one keeper starts.
Anthony Martial, Paul Pogba and Scott McTominay are all available after missing Sunday's trip to St Mary's, with Martial starting in attack alongside Marcus Rashford, Edinson Cavani and Bruno Fernandes.
McTominay partners Fred in midfield, while Pogba returns to the bench.
United only need a point from their remaining two Group H matches to secure progress to the knockout phase, but Solskjaer wants more.
"The quicker you can qualify the better, of course," he said. "With a win, we'll win the group so that's our intent.
"We want to go out there in our style, attacking intent, defending well against a top team with some top individual players, of course.
"But our mindset is win this game and we've won the group."
Man Utd XI: De Gea; Wan-Bissaka, Lindelof, Maguire, Telles; Fred, McTominay; Rashford, Bruno Fernandes, Martial; Cavani
Subs: Henderson, Bailly, Pogba, Fosu-Mensah, Williams, James, Lingard, Mata, Matic, van de Beek, Greenwood, Ighalo
Confirmed Man United squad
Goalkeepers: David De Gea, Dean Henderson, Lee Grant.
Defenders: Eric Bailly, Tim Fosu-Mensah, Victor Lindelof, Harry Maguire, Alex Telles, Aaron Wan-Bissaka, Brandon Williams.
Midfielders: Bruno Fernandes, Fred, Daniel James, Jesse Lingard, Juan Mata, Nemanja Matic, Scott McTominay, Paul Pogba, Donny van de Beek.
Forwards: Edinson Cavani, Mason Greenwood, Anthony Martial, Odion Ighalo, Marcus Rashford.
Additional reporting by Press Association.