The 109-time Supercars race winner will make his debut during the sixth round of the successful virtual series on May 13, joining a growing list of high-profile wildcard entries.
The likes of Max Verstappen, Marcos Ambrose, Will Power and Joey Logano have already made cameo appearances, while Lando Norris and James Courtney are among this week's five-strong contingent of one-offs.
Motorsport.com understands Lowndes was originally slated to race in tonight's Spa-Francorchamps round, but will instead be the headline act for next Wednesday.
Round 6 is currently set to be split across the Circuit of the Americas and Sebring layouts, although Supercars has been tweaking formats along the way, such as dropping the Nürburgring from tonight's schedule.
Scott McLaughlin leads the Eseries after four rounds, holding an 80-point advantage over Cam Waters.