Juventus winger Juan Cuadrado has tested positive for coronavirus, a day after team-mate Alex Sandro, the Italian Serie A champions say.
The club said Brazilian left back Sandro showed "mild symptoms" of COVID-19 before the test. He was in isolation with Colombian winger Cuadrado.
Both will miss Wednesday's big match at league leaders AC Milan, while team and staff entered a bubble prescribed to continue training and playing.
Juve was already the underdog going into Wednesday's match before its two team members became sick.
AC Milan have led Serie A for weeks as they aim to end the long reign of champions Juventus who they face in an intriguing duel as part of a full 10-match programme.
Juve have failed to dominate so far under new coach Andrea Pirlo.
Milan top the table on 37 points from 15 games with Juve fifth on 27, although they do have a game in hand.
Juve's Cristiano Ronaldo and Weston McKennie tested positive for coronavirus in October. Ronaldo missed two games while McKennie had to sit one out.
Daniele Rugani and Blaise Matuidi, who have both left Juve, as well as Paulo Dybala suffered the infection last season in March and April.