Samir Handanovic has become the second Inter Milan player this week to test positive for COVID-19.
The 36-year-old Inter captain and goalkeeper will be unavailable for their Serie A game against Sassuolo, the league leaders said on Wednesday.
Handanovic's positive test came a day after full back Danilo D'Ambrosio was found to have contracted the virus.
A team news conference scheduled for Friday has been postponed due to concerns about further cases.
Slovenian Handanovic has played every game, across all competitions, for Inter this season, playing a key role in helping Antonio Conte's side move into a strong position to win a first league title in 11 years.
Inter go into the weekend nine points clear of second-placed rivals AC Milan at the top of the standings having played 27 games, and 10 ahead of champions Juventus in third.
Meanwhile, Spain's top health official has rejected the idea of having fans in the stands for the Copa del Rey final between Basque Country rivals Athletic Bilbao and Real Sociedad.
Health Minister Carolina Darias said it wouldn't be appropriate "considering the situation we are in" to allow fans to attend the April 3 final in Sevilla.
Spectators have been barred from elite Spanish soccer matches since March 2020 because of the pandemic.
Subsequently, the 2020 Copa final was postponed from last year in the hope that fans from the fierce rivals could attend.
This season's final between Barcelona and Athletic will be played on April 17.
Spanish league president Javier Tebas said earlier on Wednesday he hoped to have some fans back for league games after Easter.
In France, clubs from the country's top two divisions will not release players called up for their national teams if the teams are playing outside the European Union (EU) due to the strict COVID-19 quarantine rules.
The two-week international break begins after the weekend's domestic games when European national teams are set to kick off their World Cup qualifying campaigns.
FIFA said last month that clubs are not expected to release players if there is a "mandatory period of quarantine or self-isolation of at least five days upon arrival" in the country where the club is located or the country where the match is taking place.
France host Ukraine on March 24 in Paris before playing away to Kazakhstan on March 28 and then Bosnia and Herzegovina three days later.
Les Bleus coach Didier Deschamps is set to name his squad on Thursday.