While cyclist Amanda Reid topped the podium, the Paralympic gold medal prospects for Australia's wheelchair rugby team don't look promising after another loss.
Reid won Australia's sole gold medal on Friday - the seventh for the Games - with four silver and eight bronze, leaving the national team fourth on the Tokyo medal table behind runaway leaders China.
Reid went one better than her 500m time trial silver medal at the 2016 Rio Games, dominating the C1-3 event.
The three-time Paralympian - she swam in London - clocked a world record time of 38.487 seconds to collect Australia's third cycling gold medal at the Games.
Teammate Alistair Donohoe chimed in with a repeat of his Rio silver medal in the C5 4000m individual pursuit.
The Steelers scraped into the wheelchair rugby semi-finals despite a comprehensive 57-53 loss to hosts Japan on Friday, their second group defeat.
Going for a gold medal three-peat, the Australian team's quest looks on shaky ground although skipper Ryley Batt vowed to be "sharper" in their final-four match on Saturday.
"We were very flat and not hungry enough and that's not us - usually Australia are a lot fiercer than that," Batt said.
"We'd never lost at the Paralympics since 2008 so it's a bit weird but we haven't played at this intensity for two years so this is fantastic learning for us.
"We'll be a different team tomorrow, a lot sharper. We had some sharp moments in that game but we also had some pretty bad moments."
Sprinter Isis Holt broke through to win Australia's first track and field medal, a silver after he was beaten home by China's Zhou Xia in the T35 100m.
In a repeat of their Rio results, Zhou got off to a flying start to break Holt's world record by 0.43 seconds, crossing in 13.00 seconds.
Holt, 20, is already eyeing revenge in the 200 metres on Sunday.
"It got nice and close at the end and if we'd had another 50m then who knows," the Victorian said.
"It would be great to get that gold. I know my 200m is strong and that's the event I'm excited to run."
Australia looked in good shape to grab a medal in the men's F38 javelin but Colombian Jose Lemos needed just one throw to break Queenslander Corey Anderson's world record and win gold.
Anderson just missed out on a medal despite a season's best throw, bumped into fourth while 2017 world champion Jayden Sawyer finished seventh.
The Rollers continued their strong start in men's wheelchair basketball, easily beating Algeria 83-37, but have some testing match ahead including Germany and the United States.
In swimming, Sydneysider Tiffany Thomas Kane won bronze in a thrilling finish to the SM7 200 metres individual medley.
While American Mallory Weggemann was a clear winner, just 0.34 seconds separated the next three swimmers.
Queensland's Katja Dedekind managed to hold on for bronze in the women's 400 metres freestyle (S13) after also placing third in the 100m backstroke. She also won bronze in Rio.