Gwalia Lightning will look to maintain their unbeaten home record when they host Edinburgh in the Celtic Challenge at Cardiff Arms Park on Sunday.
Lightning dropped to second in the six-team table after defeat to Irish side Clovers last time out.
Wales' other team Brython Thunder are still in search of their first win of the competition.
They travel to Kingspan Stadium in Belfast to take on top of the table Wolfhounds on Saturday.
Bryonie King once again captains Lightning, while Wales fly-half Robyn Wilkins is set to make her debut having been a late withdrawal in round three due to illness.
Tess Evans, from Australia, is also set for a first outing at number eight.
Nel Metcalfe, Gwennan Hopkins and Catherine Richards have all been recalled by Gloucester-Hartpury.
Gwalia Lightning head coach Cat Nicholas-McLaughlin said: "We know if we stick to our game plan and structures we are a match for any team in the Celtic Challenge.
"We intend to build on our impressive performances so far and there were lessons for us to take on board in our last game out in Ireland."
Thunder face a stern test out in Belfast, but take confidence from an improved performance against Edinburgh at Parc y Scarlets.
Head coach Ashley Beck has reshuffled his backline, with Lowri Williams coming in at full-back and Eleanor Hing moving to the wing.
Seren Singleton replaces Meg Davies at scrum-half, while the forwards are unchanged.
"We made significant strides against Edinburgh and look like a team with real potential and threats with the ball in hand," said Beck.
"We scored some good tries, but we know how tough the Wolfhounds will be at the Kingspan.
"They are the top of the table side and have won all three games so far in the tournament, but this is the type of challenge we relish, and we are looking forward to another improved performance away from home."
Brython Thunder team to face Wolfhounds: Lowri Williams; Eleanor Hing, Ellie Tromans, Meg Webb, Amy Williams; Niamh Terry, Seren Singleton; Chloe Thomas-Bradley, Rosie Carr, Cadi Lois Davies, Natalia John, Shona Wakley, Finley Jones, Alex Callender (captain), Sioned Harries.
Replacements: Amy Morgan, Madi Johns, Katie Carr, Charlie Mundy, Katie Mackay, Katie Bevans, Mollie Wilkinson, Sian Davies.
Gwalia Lightning team to face Edinburgh Rugby: Rhodd Parry; Caitlin Lewis, Jenna De Vera, Rebecca De Filippo, Kate Davies; Robyn Wilkins, Sian Jones; Abbey Constable, Molly Reardon, Jenni Scoble, Paige Jones, Bryonie King (capt), Maisie Davies, Lucy Isaac, Tess Evans.
Replacements: Sophie Waugh, Danyelle Dinapoli, Lowri Williams, Erin Jones, Sydney Mead, Kierra Deeks, Rachel Thomas Evans, Katie Thicker.