Pieter-Steph du Toit will play on the flank while 'Beast' Tendai Mtawarira becomes the sixth Springbok to reach 100 caps, with South Africa naming their team to face England in the second Test in Bloemfontein.
The versatile Du Toit, who has won 22 of his 34 caps at flank and has also previously played number eight, comes in for Jean-Luc du Plessis, who drops to the bench.
Du Toit will be a formidable extra jumper in the line-outs and is also a powerful runner with the ball in hand as the Boks seek to wrap-up the three-game series after winning the thrilling first Test 42-39.
The only other change to the starting lineup sees tight-head prop Frans Malherbe return from long-term injury in place of Trevor Nyakane.
Elton Jantjies has been axed from the bench which leaves no natural back-up to starting flyhalf Handre Pollard.
Much of the focus from the home support will be on Zimbabwe-born prop Mtawarira, affectionately known as 'Beast', who joins a select band of Boks to have played 100 internationals.
That list also includes former lock Victor Matfield (127 caps), winger Bryan Habana (124), hooker John Smit (111), centre Jean de Villiers (109) and fullback Percy Montgomery (102).
"Much like a typical front rower, Beast is not someone who likes the spotlight," coach Rassie Erasmus said on Thursday.
"However, his contribution to Springbok rugby during the past decade has been enormous and I want to congratulate him on reaching 100 test caps for South Africa."3.
Willie le Roux, S'busiso Nkosi, Lukhanyo Am, Damian de Allende, Aphiwe Dyantyi, Handre Pollard, Faf de Klerk, Duane Vermeulen, Pieter-Steph du Toit, Siya Kolisi (capt), Franco Mostert, RG Snyman, Frans Malherbe, Bongi Mbonambi, Tendai Mtawarira
Replacements: Akker van der Merwe, Steven Kitshoff, Thomas du Toit, Jean-Luc du Plessis, Sikhumbuzo Notshe, Ivan van Zyl, Jesse Kriel, Warrick Gelant