The Catalonian side took to the pitch wearing special shirts featuring the iconic logo of the legendary rock group - the "hot lips".
The special shirts were also on sale.
Band members Mick Jagger and Ronnie Wood were both at the game as the hosts vied to hurdle their opponents in the Spanish league.
Jagger, 80, and Wood, 76, were spotted cheering in the stands at Lluis Companys Olympic Stadium, where Barcelona are playing their home games while the Camp Nou is being renovated.
Fellow band member Keith Richards did not appear to be at the match.
Stones' drummer Charlie Watts died in August 2021 at the age of 80.
Barcelona also honoured the British band ahead of kick-off by displaying a mosaic of burgundy and blue with a huge Rolling Stones logo.
Their tribute comes in the same week the group's new album Hackney Diamonds claimed top spot in the UK charts -drawing them level with The Beatles, Bruce Springsteen and Robbie Williams for the record for most number one studio albums.
Meanwhile, Barcelona lost the El Clasico game in the dying embers to their rivals as Englishman Jude Bellingham scored twice.
The club, which has a sponsorship deal with audio-streaming service Spotify, has worn special shirts for recent Clasicos featuring the logos of Spanish pop star Rosalía and Canadian rapper Drake.