The Australian men's road cycling team will be one rider short at the Tokyo Olympics.
While the women will have a full quota of four riders to support Amanda Spratt's gold medal tilt, the men missed out on having five berths for their road race.
Cycling's world governing body the UCI has announced the national Olympic road quotas, with 130 starters in the men's event on July 25 and 67 women racing the next day.
Belgium, Colombia, France, Italy and the Netherlands secured five starters apiece in the 234km men's road race, while Australia are among seven countries who have four riders.
Australia will also have two berths in the individual time trial, where Rohan Dennis is the defending world champion.
Spratt, who won bronze at the world road championships in September, is among the favourites for the 137km women's road race.
The powerful Dutch women's team will also have four berths, as do Germany, Italy and the United States.
Australia will have two women's time trial starts, but Kat Garfoot's retirement means the event is less of a focus.
The men's 44.2km time trial and women's 22.1km event will be held on July 29.