A magnitude-4.3 earthquake has struck south of Moe in Gippsland.
The tremor was also felt in parts of Melbourne.
The are no reports of damage.
A separate 5.4-magnitude earthquake hit the area on June 19.
Geoscience Australia seismologist Jonathan Bathgate says it is hard to predict if more large aftershocks will follow.
"After events such as the magnitude 5.4, we usually see a tailing off of activity and they tend to become less frequent and smaller," he said.
"Certainly this is the largest aftershock that we've recorded since the magnitude 5.4 last month, although there have been well over 200 aftershocks since that event, but this one is the largest," he said.
Morwell resident Edward Callega says he knew something was up when his sliding door began to rattle.
"I was) talking on the phone, a wall phone, and adjacent to a sliding door and the door swung in the cavity from side to side," he said.
"I thought, oh gosh, here we go again."